کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2594785 1132281 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of blue-green algal toxin cylindrospermopsin (CYN) on human granulosa cells in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Effects of blue-green algal toxin cylindrospermopsin (CYN) on human granulosa cells in vitro
چکیده انگلیسی

The blue-green algal toxin cylindrospermopsin (CYN) occurs in public water supplies. CYN was hepatotoxic when administered orally to mice, and cytotoxic and genotoxic to human cell lines. To determine the effects of CYN on primary human IVF-derived granulosa cells, 0–1 μg/ml CYN was added to cells for 2, 4 or 6 h ± hCG (n = 6), or for 24, 48 and 72 h (n = 6). Cytotoxicity was evaluated by MTT assay, and secreted progesterone or estrogen quantified by radioimmunoassay. 24 h exposure to 1 μg/ml CYN was cytotoxic (p < 0.05), whereas 0.0625 μg/ml CYN did not cause cytotoxicity or affect estrogen production, but did inhibit basal progesterone production (p < 0.01). Similarly, 6 h exposure to 1 μg/ml CYN did not affect cytotoxicity or hCG-stimulated estrogen production, but did inhibit hCG-stimulated progesterone production (p < 0.01). In this in vitro assay, CYN inhibited progesterone production and therefore has the potential to be an endocrine disrupter by changing the progesterone:estrogen ratio in women.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 25, Issue 3, April 2008, Pages 374–380
نویسندگان
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