کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2595251 1132302 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo and in vitro effects of flutamide and diethylstilbestrol on fetal testicular steroidogenesis in the rat
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
In vivo and in vitro effects of flutamide and diethylstilbestrol on fetal testicular steroidogenesis in the rat
چکیده انگلیسی

Prenatal testosterone surge is considered crucial for physiological masculinization of male progeny. Disorders in sex steroid hormone balance during the fetal development may interfere with male reproductive health later in life. In this study, we have investigated in utero and in vitro effects of flutamide (FLU) and diethylstilbestrol (DES) on fetal rat testicular steroidogenesis. In utero exposure to FLU 25 mg/kg or DES 0.02 mg/kg had no obvious effects on ED 19.5 rat testicular testosterone and progesterone production, StAR protein or AR protein expression. However, when ED 19.5 rat testis were cultured for 180 min in the presence of 0.1, 1, 10 and 100 mg/l of FLU or DES, the highest doses of both compounds were capable of disturbing steroidogenesis. To study the rate of the changes seen in testicular steroidogenesis after 180 min, time-series experiments, in which intact testes were cultured with FLU 100 mg/l or DES 100 mg/l for 30, 60 or 120 min, were performed. In vitro time-series experiments revealed that changes in steroidogenesis occur very fast. Experiments with FLU brought further evidence to the hypothesis that ARs have negative autocrine role in developing Leydig cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 25, Issue 1, January 2008, Pages 76–83
نویسندگان
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