کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2596112 1562378 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hepatoprotective activity of berberine is mediated by inhibition of TNF-α, COX-2, and iNOS expression in CCl4-intoxicated mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Hepatoprotective activity of berberine is mediated by inhibition of TNF-α, COX-2, and iNOS expression in CCl4-intoxicated mice
چکیده انگلیسی
This study investigated the protective effects of isoquinoline alkaloid berberine on the CCl4-induced hepatotoxicity in mice. Berberine was administered as a single dose at 5 and 10 mg/kg intraperitoneally (i.p.), 1 h before CCl4 (10%, v/v in olive oil, 2 ml/kg) injection and mice were euthanized 24 h later. The rise in serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in CCl4-intoxicated mice was markedly suppressed by berberine in a concentration-dependent manner. The decrease in hepatic activity of superoxide dismutase (Cu/Zn SOD) and an increase in lipid peroxidation were significantly prevented by berberine. Histopathological changes were reduced and the expression of tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) was markedly attenuated by berberine 10 mg/mg. The results of this study indicate that berberine could be effective in protecting the liver from acute CCl4-induced injury. The hepatoprotective mechanisms of berberine may be related to the free radical scavenging and attenuation of oxidative/nitrosative stress, as well as to the inhibition of inflammatory response in the liver.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 280, Issues 1–2, 4 February 2011, Pages 33-43
نویسندگان
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