کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2597463 | 1562420 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The novel histone deacetylase inhibitor 4-Me2N-BAVAH differentially affects cell junctions between primary hepatocytes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
TSATBSHDACPBSGJICSAHAgap junctional intercellular communication - ارتباط بین سلولی بین دو سلولیSuberoylanilide hydroxamic acid - اسید سدئروانیلید هیدروکسامTrichostatin A - تریکوستاتین Agap junction - فاصله ی شکافlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Tris-buffered saline solution - محلول شور نمک Trisphosphate-buffered saline solution - محلول نمک فسفات بافرhistone deacetylase inhibitor - مهار کننده هیستون داسیدلازPrimary hepatocytes - هپاتوسیتهای اولیهhistone deacetylase - هیستون داستیلازAdherens junction - پیوند پیوندهاconnexin - کنسکسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Histone deacetylase (HDAC) inhibitors show great pharmaceutical potential, particularly in relation to cancer. However, very little is known about their biological outcome on hepatocytes, the major executors of xenobiotic biotransformation in the organism. The current study was set up to investigate the effects of the newly synthesized HDAC inhibitor 5-(4-dimethylaminobenzoyl)-aminovaleric acid hydroxamate (4-Me2N-BAVAH) on hepatocyte gap junctions and adherens junctions, being main guardians of liver homeostasis. For that purpose, freshly isolated rat hepatocytes were cultivated for 7 days either in the absence or presence of 50 μM 4-Me2N-BAVAH. Gap junction activity became promoted upon exposure to 4-Me2N-BAVAH, which was associated with elevated Cx32 protein levels. By contrast, both Cx26 and Cx43 protein levels were negatively affected. The modifications in connexin protein content were not reflected at the transcriptional level. Finally, neither the expressions nor the cellular localizations of the adherens junction building stones E-cadherin, β-catenin and γ-catenin were altered by 4-Me2N-BAVAH, a finding that is in contrast to what is commonly observed in tumor cells following exposure to HDAC inhibitors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 236, Issues 1â2, 1 July 2007, Pages 92-102
Journal: Toxicology - Volume 236, Issues 1â2, 1 July 2007, Pages 92-102
نویسندگان
Mathieu Vinken, Tom Henkens, Sarah Snykers, Aneta Lukaszuk, Dirk Tourwé, Vera Rogiers, Tamara Vanhaecke,