کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2598676 1133146 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cynaropicrin attenuates UVB-induced oxidative stress via the AhR–Nrf2–Nqo1 pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Cynaropicrin attenuates UVB-induced oxidative stress via the AhR–Nrf2–Nqo1 pathway
چکیده انگلیسی


• Artichoke is known for its antioxidant and anti-inflammatory activities.
• Cynaropicrin is a major bioactive phytochemical in the artichoke.
• Cynaropicrin activates AhR–Nrf2–Nqo1 antioxidant pathway.
• Antioxidant cynaropicrin attenuates ultraviolet B-induced oxydation in keratinocytes.
• Antioxidant cynaropicrin potently inhibits the production of IL-6 and TNFα.

Due to its antioxidant and anti-inflammatory activities, artichoke (Cynara scolymus) has been used as folk medicine to treat various diseases. Cynaropicrin (Cyn), a sesquiterpene lactone, is the major bioactive phytochemical in the artichoke; however, its pharmacological mechanism remains unknown. Because some phytochemicals exert their antioxidant activity by activating aryl hydrocarbon receptor (AhR), leading to subsequent induction of the antioxidant pathway including nuclear factor E2–related factor 2 (Nrf2) and NAD(P)H:quinone oxidoreductase 1 (Nqo1), we investigated whether Cyn also activates the AhR–Nrf2–Nqo1 pathway. Cyn indeed induced the activation (nuclear translocation) of AhR, leading to nuclear translocation of Nrf2 and dose-dependent upregulation of Nrf2 and Nqo1 mRNAs in human keratinocytes. The Cyn-induced AhR–Nrf2–Nqo1 activation was AhR- and Nrf2-dependent, as demonstrated by the observation that it was absent in keratinocytes transfected by siRNA against either AhR or Nrf2. In accordance with these findings, Cyn actively inhibited generation of reactive oxygen species from keratinocytes irradiated with ultraviolet B (UVB) in a Nrf2-dependent manner. Cyn also inhibited the production of proinflammatory cytokines such as interleukin 6 and tumor necrosis factor-α from UVB-treated keratinocytes. Our findings demonstrate that Cyn is a potent activator of the AhR–Nrf2–Nqo1 pathway, and could therefore be applied to prevention of UVB-induced photo aging.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 234, Issue 2, 16 April 2015, Pages 74–80
نویسندگان
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