کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2599341 1133200 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein carbonylation in human endothelial cells exposed to cigarette smoke extract
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Protein carbonylation in human endothelial cells exposed to cigarette smoke extract
چکیده انگلیسی

Cigarette smoke is a significant independent risk factor for vascular diseases and is a leading cause of structural and functional alterations of the vascular endothelium. In this study, we show protein carbonylation in the human umbilical vein endothelial cell line (ECV-304) exposed to whole-phase cigarette smoke extract. The main carbonylated proteins, including cytoskeletal proteins, glycolytic enzymes, xenobiotic metabolizing and antioxidant enzymes, and endoplasmic reticulum proteins, were identified by means of two-dimensional electrophoresis and Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry (redox proteomics). Morphological analyses by fluorescence microscopy evidenced alterations in the microtubule cytoskeleton, especially at longer exposure time to cigarette smoke extract. Morphological analyses by transmission electron microscopy showed vacuolisation of the cytoplasm, alteration of mitochondria ultrastructure, and some enlargement of the perinuclear space. The possible role played by protein carbonylation caused by reactive species contained in cigarette smoke in the cigarette smoke-induced endothelial injury is discussed.


► We show protein carbonylation in human endothelial cells exposed to cigarette smoke.
► The main carbonylated proteins were identified by means of redox proteomics.
► The main carbonylated proteins are cytoskeletal proteins, glycolytic enzymes, Hsp90.
► Fluorescence microscopy evidenced cytoskeletal alterations.
► TEM showed cytoplasm vacuolisation and alteration of mitochondria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 218, Issue 2, 12 April 2013, Pages 118–128
نویسندگان
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