کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2600080 1562640 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
An in vitro model to evaluate the inflammatory response after gaseous formaldehyde exposure of lung epithelial cells
چکیده انگلیسی

Asthma is a public health problem worldwide, and indoor air pollution considered to be a potential etiology. New tools need to be developed to study the effects of air pollutants in vitro and modelize inhalation exposure. This study was thus set up to design an in vitro model, using a direct exposure device to study the cellular effects of air pollutants at environmental doses on lung epithelial cells, and apply this to gaseous formaldehyde (FA). A549 cells were exposed using the direct exposure device (air/liquid interface) to FA without, after and before TNFα (1 ng/mL) sensitization. 24 h after exposure, cellular viability (XTT) and inflammation (IL-6, IL-8 and MCP-1) were assessed. No effects on cellular viability were observed for concentrations ≤50 μg/m3. After TNFα sensitization, FA-exposure induced a significant increase in IL-8 (p < 0.001), which could lead to the initiation or pathogenesis of non-specific respiratory inflammation. The results of this study demonstrate the feasibility and sensitivity of the exposure system for testing inflammatory cellular effects of indoor gaseous compounds at environmental doses directly on human respiratory cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 195, Issues 2–3, 2 June 2010, Pages 99–105
نویسندگان
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