کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2600088 1562640 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Serum proteomic profiling analysis of chronic arsenic exposure by using SELDI-TOF-MS technology
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Serum proteomic profiling analysis of chronic arsenic exposure by using SELDI-TOF-MS technology
چکیده انگلیسی

This study was to investigate the differences in serum proteomic profiling among subjects exposed to different levels of arsenic in drinking water and to identify proteins related to arsenicosis. A total of 148 subjects from endemic regions of China were selected and divided into low (0–8.30 μg/L), medium (16.30–37.60 μg/L) and high (140.88–273.67 μg/L) exposure groups. High exposure group consisted of subjects with and without skin lesions. Serum proteomic profiling was analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology. Twenty proteins were found significantly different among three groups. Further multivariate logistic regression revealed that no different proteins existed in medium versus low exposure group. A panel of five proteins, including m/z of 15167.7, 7783.1, 7580.7, 2952.6 and 2237.4, was seen in high versus low exposure group, with a sensitivity of 93.0% and specificity of 87.5%. The same panel was found in high versus a combination of low and medium exposure groups but with somewhat lower predicting power. Only one protein was significantly different between high exposure group with and without skin lesions. The results indicate that high levels of arsenic exposure could significantly change human serum proteomic profiling, which can be detected before skin lesions occur.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 195, Issues 2–3, 2 June 2010, Pages 155–160
نویسندگان
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