کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602280 1133386 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antioxidants do not prevent acrylonitrile-induced toxicity
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Antioxidants do not prevent acrylonitrile-induced toxicity
چکیده انگلیسی

Several reports have recently described that acrylonitrile (ACN) toxicity resides in its capacity for inducing oxidative stress. ACN can be conjugated with glutathione (GSH), diminishing its cellular content, or being metabolized to cyanide. In the present report, we determine the effect of ACN on the viability of primary-cultured astrocytes as well as the oxidative damage generated by ACN by measuring GSH levels in primary cultured astrocytes. We also analyzed whether the ACN (2.5 mM) toxicity could be avoided by using antioxidants such as taurine (5 mM), N-acetylcysteine (20 mM), trolox (100 μM), estradiol (10 μM) and melatonin (100 nM–1 mM). In this cell culture model, antioxidants were not able to prevent ACN-induced cell damage, with the exception of NAC, confirming that only GSH seems to play a key role in ACN-derived toxicity. Additionally, we measured different parameters of oxidative stress such as catalase activity, lipid peroxidation and GSH concentration, as indicators of the potential oxidative stress mediated by the toxicity of ACN, after exposure of Wistar rats to a concentration of 200 ppm ACN for 14 days. At the concentration assayed, we did not find any evidence of oxidative damage in the brain of ACN-treated rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 169, Issue 3, 30 March 2007, Pages 236–244
نویسندگان
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