کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602375 1133414 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The inhibitory effects of deltamethrin on dopamine biosynthesis in rat PC12 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
The inhibitory effects of deltamethrin on dopamine biosynthesis in rat PC12 cells
چکیده انگلیسی

The inhibitory effects of deltamethrin (DM) on dopamine biosynthesis in PC12 cells were investigated. Levels of dopamine in PC12 cells were detected after treatment with different doses from 10−4 M to 10−6 M deltamethrin or combined with 20 μM or 50 μM l-3,4-dihydroxyphenylalanine (l-Dopa) for 3 h or 12 h by HPLC. Flow cytometry (FCM) and RT-PCR technology were used to detect the levels of tyrosine hydroxylase (TH) mRNA and protein in PC12 cells treated with different doses of deltamethrin for 3 h or 12 h. Deltamethrin decreased the levels of dopamine, TH mRNA and TH protein in PC12 cells, and the increased dopamine levels induced by l-Dopa at 20–50 μM were also decreased by deltamethrin for 3 h or 12 h. These results indicate that deltamethrin decreases dopamine content by the inhibition of TH mRNA and protein level in PC12 cells and maybe also have inhibitory effect on aromatic l-amino acid decarboxylase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 161, Issue 3, 1 March 2006, Pages 195–199
نویسندگان
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