کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2602492 | 1133762 | 2015 | 7 صفحه PDF | دانلود رایگان |

• Mechanisms of hyperforin and aristoforin DNA-protective activities were studied in vitro.
• Both chemicals protect DNA against Fe2+-induced DNA breaks.
• Mechanism of DNA protection is based on their activity to scavenge free radicals, mainly OH.
• Hyperforin and aristoforin are not able to inhibit topoisomerase I.
• Hyperforin and aristoforin could not intercalate into DNA.
The aim of this study was to explain the molecular mechanisms of action of hyperforin, a phluoroglucinol derivative found in Hypericum perforatum L. and its more stable derivative aristoforin. DNA-topology assay revealed partial DNA-protective activities of hyperforin and aristoforin against Fe2+-induced DNA breaks. In order to assess molecular mechanisms underlying DNA-protective activity, the potential antioxidant activity of hyperforin and aristoforin was investigated using DPPH and OH scavenging assays, reducing power assay and Fe2+-chelating assay. We also studied interaction of hyperforin and aristoforin with DNA using established protocols for fluorescence titration. The ability of the studied compounds to relax topoisomerase I with electrophoretic techniques was investigated. The reduction in the fluorescence of hyperforin indicated an interaction between hyperforin and DNA with a binding constant of 0.2 × 108 M−1. We suggest that a mechanism of hyperforin/aristoforin DNA-protective abilities is based on free radicals (mainly OH) scavenging activity.
Journal: Toxicology in Vitro - Volume 29, Issue 3, April 2015, Pages 631–637