کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602492 1133762 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA-protective activities of hyperforin and aristoforin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
DNA-protective activities of hyperforin and aristoforin
چکیده انگلیسی


• Mechanisms of hyperforin and aristoforin DNA-protective activities were studied in vitro.
• Both chemicals protect DNA against Fe2+-induced DNA breaks.
• Mechanism of DNA protection is based on their activity to scavenge free radicals, mainly OH.
• Hyperforin and aristoforin are not able to inhibit topoisomerase I.
• Hyperforin and aristoforin could not intercalate into DNA.

The aim of this study was to explain the molecular mechanisms of action of hyperforin, a phluoroglucinol derivative found in Hypericum perforatum L. and its more stable derivative aristoforin. DNA-topology assay revealed partial DNA-protective activities of hyperforin and aristoforin against Fe2+-induced DNA breaks. In order to assess molecular mechanisms underlying DNA-protective activity, the potential antioxidant activity of hyperforin and aristoforin was investigated using DPPH and OH scavenging assays, reducing power assay and Fe2+-chelating assay. We also studied interaction of hyperforin and aristoforin with DNA using established protocols for fluorescence titration. The ability of the studied compounds to relax topoisomerase I with electrophoretic techniques was investigated. The reduction in the fluorescence of hyperforin indicated an interaction between hyperforin and DNA with a binding constant of 0.2 × 108 M−1. We suggest that a mechanism of hyperforin/aristoforin DNA-protective abilities is based on free radicals (mainly OH) scavenging activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 29, Issue 3, April 2015, Pages 631–637
نویسندگان
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