کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602824 1133799 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mononaphthalimide spermidine conjugate induces cell proliferation inhibition and apoptosis in HeLa cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Mononaphthalimide spermidine conjugate induces cell proliferation inhibition and apoptosis in HeLa cells
چکیده انگلیسی

Developing polyamine-drug conjugates that are capable of specific entry to tumor cells is attractive in improving chemotherapeutic efficacy. Currently, the exact cytotoxic mechanism of these conjugates is not well known. Here, our research revealed the effect of a mononaphthalimide-spermidine (MNISpd) conjugate on the growth and survival of HeLa cells and possible mechanisms. In characterizing the mechanism of MNISpd cytotoxicity, inhibition of proliferation is observed in the 0.5–6 μM range and there is evidence of apoptosis at equal or greater than 6 μM, but with less toxicity on HELF cell. The lower concentrations of MNISpd induced a cell cycle arrest correlated with enhanced p21 expression and decreased cdc2 but not Cdk2 expression. MNISpd -induced apoptosis was correlated with caspase-3 activation, decreased XIAP expression and a loss of mitochondrial membrane potential. Apoptosis but not cell cycle arrest was susceptible to N-acetyl-l-cysteine (NAC) treatment. It is proposed that MNISpd-induced apoptosis in HeLa cells is related to oxidative stress and that at lower exposure concentrations effects on cell proliferation predominate while at higher concentrations apoptosis develops.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 25, Issue 4, June 2011, Pages 882–889
نویسندگان
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