کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2787935 1154367 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells
چکیده انگلیسی

Induced pluripotent stem (iPS) cells can be derived from human somatic cells by cellular reprogramming. This technology provides a potential source of non-controversial therapeutic cells for tissue repair, drug discovery, and opportunities for studying the molecular basis of human disease. Normally, mouse embryonic fibroblasts (MEFs) are used as feeder layers in the initial derivation of iPS lines. The purpose of this study was to determine whether SNL fibroblasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors. In our study, iPS cells expressed common pluripotency markers, displayed human embryonic stem cells (hESCs) morphology and unmethylated promoters of NANOG and OCT4. These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetics and Genomics - Volume 37, Issue 4, April 2010, Pages 241–248
نویسندگان
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