کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2811308 | 1158685 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Experimental Study on the Suppression of Human Nuclear Receptor hLRH-1 via a Vector-based RNA Interference Experimental Study on the Suppression of Human Nuclear Receptor hLRH-1 via a Vector-based RNA Interference](/preview/png/2811308.png)
To explore the inhibitions of human nuclear receptor hLRH-1 via RNA interference, siRNAs expressing vectors pShLRH-1.1 and pShLRH-1.2, and targeting hLRH-1 were designed and constructed. The recombinants were introduced into hepatocellular carcinoma cells, BEL-7402, mediated by lipofectaminTM. RT-PCR was carried out to examine the inhibition ratio of hLRH-1 expression. The same method was also applied to analyze the expression of farnesyl pyrophosphate synthetase (FPPS) gene. Our results demonstrated that after transient transfection, both pShLRH-1.1 and pShLRH-1.2 could trigger the efficient inhibition of hLRH-1 in cultured cells, BEL-7402. The inhibition ratios were up to 80%. By comparing with non-transfection and vector-transfection control, the expression of FPPS in cells with inhibition of hLRH-1 was up-regulated significantly. Thus, the inhibition of expression of hLRH-1 in cultured cells was achieved via RNA interference in this study. Our results also suggested that hLRH-1 acts as a negative regulator in FPPS expression.
Journal: Acta Genetica Sinica - Volume 33, Issue 1, January 2006, Pages 26-31