کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2818342 | 1160045 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and polymorphism analysis of the prion protein gene in Tan sheep of Ningxia, China
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کلمات کلیدی
BSEARRARQSNPsPrPTSEARHCJDPrPcPRNPScrapie - اسکرپیTransmissible spongiform encephalopathies - انسفالوپاتی های اسپگوماییک قابل انتقالCreutzfeldt–Jakob disease - بیماری Creutzfeldt-JakobBovine spongiform encephalopathy - جنون گاویMolecular cloning - همسانه سازی مولکولی، کلونینگ مولکولیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPrion protein - پروتئین پریونcellular prion protein - پروتون پریون سلولیSingle nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدیPrion protein gene - ژن پروتئین پریون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular cloning and polymorphism analysis of the prion protein gene in Tan sheep of Ningxia, China Molecular cloning and polymorphism analysis of the prion protein gene in Tan sheep of Ningxia, China](/preview/png/2818342.png)
چکیده انگلیسی
The resistance or susceptibility of sheep to scrapie is associated with polymorphisms of the prion protein gene (PRNP), particularly, single nucleotide polymorphisms (SNPs) in amino acid positions 136, 154 and 171. The prion protein (PrP) gene sequence and the deduced amino acid alignment of prion protein in Tan sheep, a local Chinese sheep breed traditionally raised in Ningxia, northwestern China, were determined and variability of the PrP amino acids sequence was analyzed in this study. The PrP nucleic acids and amino acids sequences of 112 Tan sheep were highly homogenous, although polymorphism of the PrP gene was detected at several sites, particularly codons 106, 154, and 171. The analysis of both sequences revealed that the most predominant allele at codons 136, 154 and 171 in Tan sheep was ARQ, which was known to be associated with high susceptibility to scrapie in sheep. The result suggests that Tan sheep is potentially susceptible to scrapie. Our findings provide valuable information for future breeding projects to scrapie resistance in Tan sheep.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 485, Issue 2, 10 October 2011, Pages 102-105
Journal: Gene - Volume 485, Issue 2, 10 October 2011, Pages 102-105
نویسندگان
Lihua Xu, Zhuming Zhang, Xiangmei Zhou, Xiaomin Yin, Lifeng Yang, Deming Zhao,