کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2820699 | 1160881 | 2014 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A draft genome assembly of the army worm, Spodoptera frugiperda A draft genome assembly of the army worm, Spodoptera frugiperda](/preview/png/2820699.png)
• Genomic DNA isolated from Sf21 cells was sequenced and assembled.
• The genomic assembly contains 37,243 scaffolds of size, 358 Mb with N50 of 53.7 kb.
• The Spodoptera genome contains an approximate 20.28% of repeat elements.
• 11,595 genes were predicted from the Spodoptera draft genome sequence.
• The genes were annotated and protein families unique to Spodoptera were identified.
Spodoptera is an agriculturally important pest insect and studies in understanding its biology have been limited by the unavailability of its genome. In the present study, the genomic DNA was sequenced and assembled into 37,243 scaffolds of size, 358 Mb with N50 of 53.7 kb. Based on degree of identity, we could anchor 305 Mb of the genome onto all the 28 chromosomes of Bombyx mori. Repeat elements were identified, which accounts for 20.28% of the total genome. Further, we predicted 11,595 genes, with an average intron length of 726 bp. The genes were annotated and domain analysis revealed that Sf genes share a significant homology and expression pattern with B. mori, despite differences in KOG gene categories and representation of certain protein families. The present study on Sf genome would help in the characterization of cellular pathways to understand its biology and comparative evolutionary studies among lepidopteran family members to help annotate their genomes.
Journal: Genomics - Volume 104, Issue 2, August 2014, Pages 134–143