کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2821203 | 1160930 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The probability of nonsense mutation caused by replication-associated mutational pressure is much higher for bacterial genes from lagging than from leading strands The probability of nonsense mutation caused by replication-associated mutational pressure is much higher for bacterial genes from lagging than from leading strands](/preview/png/2821203.png)
We studied nucleotide usage biases in 4-fold degenerated sites of all the genes from leading and lagging strands of 30 bacterial genomes. The level of guanine in 4-fold degenerated sites (G4f) is significantly lower in genes from lagging strands than in genes from leading strands, probably because of the faster rates of guanine oxidation in single-stranded DNA leading to G to T transversions. The rates of cytosine deamination causing C to T transitions are also higher in lagging strands. We showed that the level of codons able to form stop-codons by the way of G to T transversions and C to T transitions is always higher than the level of codons able to form stop-codons by the way of C to A transversions and G to A transitions. This circumstance can be an explanation of the lower percent of ORFs in lagging strands of bacterial replichores than in leading strands.
Journal: Genomics - Volume 96, Issue 3, September 2010, Pages 173–180