کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2821620 1160970 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple
چکیده انگلیسی

A genome-wide BAC physical map of the apple, Malus × domestica Borkh., has been recently developed. Here, we report on integrating the physical and genetic maps of the apple using a SNP-based approach in conjunction with bin mapping. Briefly, BAC clones located at ends of BAC contigs were selected, and sequenced at both ends. The BAC end sequences (BESs) were used to identify candidate SNPs. Subsequently, these candidate SNPs were genetically mapped using a bin mapping strategy for the purpose of mapping the physical onto the genetic map. Using this approach, 52 (23%) out of 228 BESs tested were successfully exploited to develop SNPs. These SNPs anchored 51 contigs, spanning ∼ 37 Mb in cumulative physical length, onto 14 linkage groups. The reliability of the integration of the physical and genetic maps using this SNP-based strategy is described, and the results confirm the feasibility of this approach to construct an integrated physical and genetic maps for apple.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 93, Issue 3, March 2009, Pages 282–288
نویسندگان
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