کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2821733 1160996 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficacy of clone fingerprinting methodologies
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Efficacy of clone fingerprinting methodologies
چکیده انگلیسی

With the development of new high-information content fingerprinting techniques for constructing BAC-based physical maps, physical map construction is accelerating and it is important to determine which methodologies work best. In a recent publication (Z. Xu et al., 2004, Genomics 84:941-951), Xu et al. evaluated five different techniques (one agarose-based and four using multiple enzymes) and concluded that a two-enzyme technique was superior. In addition, they found that no benefit was gained from fingerprinting more than 10× coverage. In this paper we report our own extensive simulation results, which lead to contrasting conclusions. Our data indicate that the five-enzyme method known as SNaPshot is the most effective and that the assembly can in fact be significantly improved with greater than 10× coverage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 89, Issue 1, January 2007, Pages 160–165
نویسندگان
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