کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2824810 | 1161866 | 2014 | 11 صفحه PDF | دانلود رایگان |
• Molecules involved in transcription have non-random spatial distributions in cells.
• Spatial organization may offer a new gene regulation mechanism at the cellular level.
• More studies are needed to correlate functionality with spatial localization patterns.
Prokaryotic transcription has been extensively studied over the past half a century. However, there often exists a gap between the structural, mechanistic description of transcription obtained from in vitro biochemical studies, and the cellular, phenomenological observations from in vivo genetic studies. It is now accepted that a living bacterial cell is a complex entity; the heterogeneous cellular environment is drastically different from the homogenous, well-mixed situation in vitro. Where molecules are inside a cell may be important for their function; hence, the spatial organization of different molecular components may provide a new means of transcription regulation in vivo, possibly bridging this gap. In this review, we survey current evidence for the spatial organization of four major components of transcription [genes, transcription factors, RNA polymerase (RNAP) and RNAs] and critically analyze their biological significance.
Journal: - Volume 30, Issue 7, July 2014, Pages 287–297