کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2832466 | 1163838 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficient clearance of opsonised apoptotic cells in the absence of PECAM-1
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کلمات کلیدی
MFINMSBMDMPECAMOpsonins - اپسونین هاApoptosis - خزان یاختهایnormal mouse serum - سرم نرمال موشphagocytic index - شاخص فاگوسیتیکPhagocytosis - فاگوسیتوز یا بیگانهخواری Bone marrow-derived macrophages - ماکروفاژها حاصل از استخوان مغز استخوانMacrophages - ماکروفاژها،درشت خوارهاplatelet endothelial cell adhesion molecule - مولکول چسبندگی سلول اندوتلیال پلاکتplatelet endothelial cell adhesion molecule-1 - مولکول چسبندگی سلول اندوتلیال پلاکتی-1mean fluorescence intensity - میانگین شدت فلورسانسwild-type - نوع وحشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
It is now accepted that the recognition and uptake of apoptotic cells by phagocytes is a complex process involving a large number of opsonins, receptors and ligands, however the relative contribution of all these molecules are still debated. Here we examined the role of CD31 (PECAM-1) in the recognition/uptake of apoptotic thymocytes by murine bone marrow-derived macrophages (BMDM) in vitro, and by resident peritoneal macrophages in vivo. In the absence of serum, CD31 deficiency, on either the phagocyte or the apoptotic cell, resulted in a reduction in the clearance of apoptotic thymocytes, when a high ratio of apoptotic cells to macrophages was applied. In the presence of serum however there was no discernible contribution of CD31 to the clearance of apoptotic cells by bone marrow-derived macrophages, irrespective of the ratio of cells used. In vivo peritoneal clearance experiments confirmed that in the presence of soluble opsonins CD31 deficiency had no effect on this process. These data suggest that the overall role played by CD31 in the ingestion of apoptotic cells is negligible and most likely overwhelmed by the effects of serum opsonins, such as complement components.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 44, Issue 6, February 2007, Pages 1135-1140
Journal: Molecular Immunology - Volume 44, Issue 6, February 2007, Pages 1135-1140
نویسندگان
Paul K. Potter, Karen Y. Larbi, Sussan Nourshargh, Marina Botto,