کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
28747 44089 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study of the interaction between doxepin and human serum albumin by spectroscopic methods
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Study of the interaction between doxepin and human serum albumin by spectroscopic methods
چکیده انگلیسی

The binding of doxepin hydrochloride (DH) to human serum albumin (HSA) was investigated by fluorescence, UV–vis absorption and circular dichroism (CD) techniques under simulative physiological conditions. The binding parameters have been evaluated by fluorescence quenching method. Negative enthalpy (ΔH°) and positive entropy (ΔS°) values indicated that both hydrogen bond and hydrophobic forces played a major role in the binding of DH to HSA. The distance r between donor (HSA) and acceptor (DH) was obtained according to the Förster's theory of non-radiation energy transfer. Spectral results revealed that the binding of DH to HSA induced conformational changes in HSA. The effect of common ions on the binding constant of DH–HSA was also examined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 179, Issues 1–2, 1 April 2006, Pages 161–166
نویسندگان
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