کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2893815 1172421 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression
چکیده انگلیسی

Background and aimsAtherosclerosis is a chronic inflammatory process involving the activity of several cytokines and growth factors. Platelet-derived growth factor-A (PDGF-A) and PDGF-B are important mitogens and chemoattractants for monocytes as well as smooth muscle cells. We sought to identify the role of PDGF-C and PDGF-D, two new members of the PDGF family, in monocyte migration and differentiation. We also assessed their effects in regulating matrix metalloproteinase-2 (MMP-2) and MMP-9, which are important for cell migration.Methods and resultsPDGF-C and PDGF-D were expressed in macrophages, smooth muscle cells, and endothelial cells in human atherosclerotic plaques, as shown by immunohistochemical analysis. PDGF-C and PDGF-D mRNA and protein expression was induced after differentiation of THP-1 monocytes to macrophages, and both PDGF-C and PDGF-D induced MMP-9 mRNA expression in a concentration-dependent manner. Treatment of cells with PDGF-C or PDGF-D enhanced the secretion of MMP-2 and MMP-9 in a cell-dependent manner. In a migration assay using a Boyden chamber with 8 μm pore size, PDGF-C and PDGF-D attracted THP-1 monocytes in a concentration-dependent manner.ConclusionsOur data suggest that PDGF-C and PDGF-D, like PDGF-A and PDGF-B, play important roles in atherosclerosis by stimulating MMP activity and influencing monocyte migration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Atherosclerosis - Volume 202, Issue 2, February 2009, Pages 415–423
نویسندگان
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