کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3001762 1180669 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impaired stimulation of glucose transport in cardiac myocytes exposed to very low-density lipoproteins
ترجمه فارسی عنوان
اختلال تحرک انتقال گلوکز در میوسیت های قلبی در معرض چربی های لیپوپروتئین بسیار کم است
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
چکیده انگلیسی


• In metabolic syndrome or type II diabetes, myocardial infarction is more severe.
• Myocardial glucose uptake stimulation determines susceptibility to ischemic damage.
• Hyperlipidemia, including increased VLDL, is a hallmark of type II diabetes.
• We investigate the direct effects of VLDL on cardiac myocytes glucose transport.
• VLDL exposure reduces basal and stimulated glucose transport in cardiac myocytes.

We recently observed that free fatty acids impair the stimulation of glucose transport into cardiomyocytes in response to either insulin or metabolic stress. In vivo, fatty acids for the myocardium are mostly obtained from triglyceride-rich lipoproteins (chylomicrons and Very Low-Density Lipoproteins). We therefore determined whether exposure of cardiac myocytes to VLDL resulted in impaired basal and stimulated glucose transport. Primary adult rat cardiac myocytes were chronically exposed to VLDL before glucose uptake was measured in response to insulin or metabolic stress, provoked by the mitochondrial ATP synthase inhibitor oligomycin. Exposure of cardiac myocytes to VLDL reduced both insulin-and oligomycin-stimulated glucose uptake. The reduction of glucose uptake was associated with a moderately reduced tyrosine phosphorylation of the insulin receptor. No reduction of the phosphorylation of the downstream effectors of insulin signaling Akt and AS160 was however observed. Similarly only a modest reduction of the activating phosphorylation of the AMP-activated kinase (AMPK) was observed in response to oligomycin. Similar to our previous observations with free fatty acids, inhibition of fatty acid oxidation restored oligomycin-stimulated glucose uptake. In conclusions, VLDL-derived fatty acids impair stimulated glucose transport in cardiac myocytes by a mechanism that seems to be mediated by a fatty acid oxidation intermediate. Thus, in the clinical context of the metabolic syndrome high VLDL may contribute to enhancement of ischemic injury by reduction of metabolic stress-stimulated glucose uptake.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition, Metabolism and Cardiovascular Diseases - Volume 26, Issue 7, July 2016, Pages 614–622
نویسندگان
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