کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3069482 | 1580684 | 2012 | 11 صفحه PDF | دانلود رایگان |

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an excessive expansion of a CAG trinucleotide repeat in the gene encoding the protein huntingtin, resulting in an elongated stretch of glutamines near the N-terminus of the protein. Here we report the derivation of a collection of 11 induced pluripotent stem (iPS) cell lines generated through somatic reprogramming of fibroblasts obtained from the R6/2 transgenic HD mouse line. We show that CAG expansion has no effect on reprogramming efficiency, cell proliferation rate, brain-derived neurotrophic factor level, or neurogenic potential. However, genes involved in the cholesterol biosynthesis pathway, which is altered in HD, are also affected in HD-iPS cell lines. Furthermore, we found a lysosomal gene upregulation and an increase in lysosome number in HD-iPS cell lines. These observations suggest that iPS cells from HD mice replicate some but not all of the molecular phenotypes typically observed in the disease; additionally, they do not manifest increased cell death propensity either under self-renewal or differentiated conditions. More studies will be necessary to transform a revolutionary technology into a powerful platform for drug screening approaches.
► We model Huntington's disease (HD) through induced pluripotent stem (iPS) cells.
► We generated a large number of iPS cell lines from a HD genetic mouse model.
► HD-iPS cells showed similar behavior in somatic reprogramming and cell cycle rate.
► Alterations in cholesterogenic genes and lysosomal biogenesis were found.
► Neurons differentiated from HD-iPS cells contained huntingtin aggregates.
Journal: Neurobiology of Disease - Volume 46, Issue 1, April 2012, Pages 30–40