Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors

► Neural progenitors are generated from N7 mES cells within 3 d of Ngn1 expression. ► N7 neural progenitors may be patterned and matured along different neural lineages. ► Ngn1-induced neural progenitors averts tumor formation after intraspinal injection. ► Grafted Ngn1-induced N7 cells differentiate into spinal cord progenitor cells.