کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3070121 | 1580714 | 2009 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The role of MMP-9 in integrin-mediated hippocampal cell death after pilocarpine-induced status epilepticus The role of MMP-9 in integrin-mediated hippocampal cell death after pilocarpine-induced status epilepticus](/preview/png/3070121.png)
Recent studies demonstrate that matrix metalloproteinase-9 (MMP-9) is closely involved in the pathogenesis of epilepsy. This study investigated the role of MMP-9 in hippocampal cell death after pilocarpine-induced status epilepticus (SE). We showed that MMP-9 expression and activity significantly increased and β1-integrin levels decreased on day 3 after SE. β1-integrin degradation was also observed in hippocampal ex vivo extracts incubated with recombinant active MMP-9. Treatment with a selective MMP-9 inhibitor attenuated MMP-9 up-regulation, β1-integrin degradation, the reduction of ILK activity and Akt phosphorylation, and subsequent hippocampal damage after SE. However, co-treatment with anti-β1-integrin antibody almost completely blocked the protective effects of the MMP-9 inhibitor on both integrin-mediated survival signaling and hippocampal cell death. Our study demonstrates that MMP-9 induces apoptotic hippocampal cell death by interrupting integrin-mediated survival signaling after SE and suggests that MMP-9 may be a promising target for a neuroprotective approach to preventing seizure-induced hippocampal damage.
Journal: Neurobiology of Disease - Volume 36, Issue 1, October 2009, Pages 169–180