کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3071 149 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capture and intermediate purification of recombinant antibodies with combined precipitation methods
ترجمه فارسی عنوان
تصفیه و تفکیک متوسط ​​از آنتی بادی های نوترکیب با روش های بارندگی ترکیبی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Combined precipitation methods with equal separation power as chromatographic methods.
• Replacement of capture and intermediate steps by caprylic acid and PEG precipitation.
• Combination of CEP, PEG, CA and CaCl2 precipitation to remove host cell impurities.
• The final yield and purity of the antibodies nearly reached drug substance values.

A protocol for capturing recombinant antibodies was developed with a combination of different precipitation methods. This process comprised four different precipitations, including caprylic acid, polyethylene glycol (PEG), CaCl2, and cold ethanol. The final yield and purity of the antibodies nearly reached drug substance values, with a low host cell protein concentration (<300 ppm), no high molecular weight impurities, and a yield of 70%. In fact, only the combination of caprylic acid and PEG precipitations was needed to achieve purity similar to that produced with protein A affinity chromatography. Caprylic acid was used to remove the host cell proteins and high molecular weight impurities, and the PEG precipitation separated the IgG from the remaining impurities.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 93, 15 January 2015, Pages 200–211
نویسندگان
, , , , , ,