کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3071011 | 1580752 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Selective nicotinic acetylcholine receptor subunit deficits identified in Alzheimer's disease, Parkinson's disease and dementia with Lewy bodies by immunoprecipitation Selective nicotinic acetylcholine receptor subunit deficits identified in Alzheimer's disease, Parkinson's disease and dementia with Lewy bodies by immunoprecipitation](/preview/png/3071011.png)
Antibodies raised against human α2-6 and β2-4 nicotinic receptor subunits were utilized to fractionate 3H-epibatidine binding in human temporal cortex and striatum. The predominant receptor subtypes in both regions contained α4 and β2 subunits. In normal cortex, 10% of binding was also associated with α2 subunits, whereas in the striatum, contributions by α6 (17%) and β3 (23%) were observed. Minimal binding (≤5%) was associated with α3.In Alzheimer's disease and dementia with Lewy bodies, cortical loss of binding was associated with reductions in α4 (50%, P < 0.01) and β2 (30–38%, P < 0.05). In Parkinson's disease and dementia with Lewy bodies, striatal deficits in α6 (91 and 59% respectively, P < 0.01) and β3 (72 and 75%, P < 0.05) tended to be greater than for α4 and β2 (50–58%, P < 0.05). This study demonstrates distinct combinations of subunits contributing to heteromeric nicotinic receptor binding in the human brain that are area/pathway specific and differentially affected by neurodegeneration.
Journal: Neurobiology of Disease - Volume 23, Issue 2, August 2006, Pages 481–489