کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3120741 1583292 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of aryl hydrocarbon receptor (AhR) in the pathology of pleomorphic adenoma in parotid gland
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
The role of aryl hydrocarbon receptor (AhR) in the pathology of pleomorphic adenoma in parotid gland
چکیده انگلیسی


• Expression of AhR system was analyzed in pleomorphic adenoma and HSY cells.
• Functional state of AhR in HSY cells was observed.
• Overexpression of AHR, ARNT, CYP1B1, and AhR epithelial and mesenchymal cell localization in pleomorphic adenoma was displayed.
• AhR system may play a role in the pathology of pleomorphic adenoma.

ObjectivesPleomorphic adenoma (benign mixed tumor) is one of the most common salivary gland tumors. However, molecular mechanisms implicated in its development are not entirely defined. Therefore, the study aimed at definition of aryl hydrocarbon receptor (AhR) involvement in pleomorphic adenoma pathology, as the AhR controlled gene system was documented to play a role in development of various human tumors.DesignThe study was carried out in pleomorphic adenoma and control parotid gland tissues where gene expression of AHR, AhR nuclear translocator (ARNT), AhR repressor (AHRR), as well as AhR controlled genes: CYP1A1 and CYP1B1, at mRNA and protein (immunohistochemistry) levels were studied. Functional evaluation of AhR system was evaluated in HSY cells (human parotid gland adenocarcinoma cells) using 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as AhR specific inducer.ResultsPleomorphic adenoma specimens showed cytoplasmic and nuclear AhR expression in epithelial cells as well as in mesenchymal cells. In parotid gland AhR was expressed in cytoplasm of duct cells. Quantitative expression at mRNA level showed significantly higher expression of AHR, ARNT and CYP1B1, and comparable levels of CYP1A1 in pleomorphic adenoma tissue in comparison to healthy parotid gland. The HSY cell study revealed significantly higher expression level of AHRR in HSY as compared with MCF-7 cells (human breast adenocarcinoma cell line used as reference). Upon TCDD stimulation a drop in AHRR level in HSY cells and an increase in MCF-7 cells were observed. The HSY and MCF-7 cell proliferation rate (measured by WST-1 test) was not affected by TCDD.ConclusionsSummarizing both in vitro and in vivo observations it can be stated that AhR system may play a role in the pathology of pleomorphic adenoma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 61, January 2016, Pages 53–59
نویسندگان
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