کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3121237 1583389 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Semaphorin 4D inhibits collagen synthesis of rat pulp-derived cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Semaphorin 4D inhibits collagen synthesis of rat pulp-derived cells
چکیده انگلیسی

ObjectiveSemaphorins are a group of secreted and membrane-associated molecules that play important roles in axon navigation. Several semaphorin family molecules are expressed in the pharyngeal arches and tooth germs. We analysed the expression of membrane-associated Semaphorin 4D (Sema4D) in tooth germs, and examined its potential role in regulating the differentiation of rat incisor pulp-derived cells in vitro.DesignmRNA expression was examined by in situ hybridisation. The effects of Sema4D on rat pulp-derived cells were examined by adenovirus-mediated overexpression in vitro.ResultsBoth epithelial and mesenchymal cells of tooth germs expressed Sema4D at the early bell stage. Later, the odontoblasts predominantly expressed Sema4D, while the epithelial expression greatly decreased. The overexpression of Sema4D in rat incisor pulp-derived cells strongly inhibited mineralisation. This inhibition was preceded by a reduction of collagen fibre production at the level of mRNA synthesis.ConclusionsThese results indicate that Sema4D is expressed in both epithelial and mesenchymal cells of the tooth germs. Sema4D represses collagen synthesis of pulp-derived cells, indicating it might negatively regulate odontoblast differentiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 53, Issue 1, January 2008, Pages 27–34
نویسندگان
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