کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3121252 | 1583373 | 2009 | 7 صفحه PDF | دانلود رایگان |

This study was designed to investigate the effects of oestrogen on sarcoplasmic reticulum (SR) Ca2+-ATPase activity and gene expression in ovariectomised rats under the condition of chronic intermittent hypoxia (CIH). Thirty-two female Sprague–Dawley rats were randomly divided into four groups: the normal control group (NC), the CIH group (CIH), the CIH–ovariectomised group (CIH + OVX), and the group of CIH–ovariectomised rats receiving estradiol replacement (CIH + OVX + E2). Rats in the latter three groups were exposed to CIH for 5 weeks. The animals were killed before genioglossus (GG) was rapidly excised, and their body and uterus mass were determined. Estradiol level was detected by radioimmunoassay. SR Ca2+-ATPase (SERCA) activity was observed by detecting inorganic phosphorus ion, and the SERCA mRNA level was measured using real-time quantitative polymerase chain reaction (real-time PCR). It was found that, compared with the NC group, the SERCA activity and mRNA level were remarkably reduced (p < .01) in the CIH group. And compared with the CIH group, the SERCA activity and mRNA level were also significantly reduced (p < .01) in the CIH + OVX group. Meanwhile, the SERCA activity and mRNA level significantly increased (p < .01) in the CIH + OVX + E2 group compared with the CIH + OVX group, but lower than those in the NC group (p < .01). The results showed that CIH could reduce the SERCA activity and mRNA expression, and oestrogen-deficiency could exacerbate this effect; whilst estradiol replacement can partially reverse the effect of CIH in ovariectomised rats.
Journal: Archives of Oral Biology - Volume 54, Issue 4, April 2009, Pages 322–328