کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3121423 | 1583354 | 2010 | 7 صفحه PDF | دانلود رایگان |

Background and objectiveA genetically diabetic mouse strain (db/db) exhibits severe obesity and a syndrome resembling human non-insulin-dependent diabetes mellitus. Our histological study of submandibular glands revealed that the size and area of the granular convoluted tubules was substantially decreased in db/db mice. We hypothesized that this structural difference reflected a specific alteration in salivary duct function.MethodsThe saliva evoked by pilocarpine was used for the measurement of ion concentrations, and submandibular glands were dissected out for the immunohistochemistry and real-time PCR study.ResultsThe K+ concentration of the salivary secretion was higher in db/db than in control m+/m+ mice, while neither saliva volume nor the concentrations of Na+ or Cl− differed between these strains. In db/db mice (vs. m+/m+ mice): quantitative PCR analysis revealed an increased mRNA expression of large-conductance Ca2+-activated K+ (maxi-K) channels, immunohistochemistry revealed an increase in the luminal surface expression of the maxi-K channel protein, and a particularly interesting finding was that there was a substantial increase in the salivary tissue-specific splice variant ParSlo.ConclusionThese results suggest that in db/db mice, the K+ content of saliva may be elevated due to an expression of a maxi-K channel variant, which results from a modification of ductal structure. General significance: Our data may shed some light on the mechanism responsible for determining the dynamics of salivary K+ concentration increased in diabetic patients.
Journal: Archives of Oral Biology - Volume 55, Issue 11, November 2010, Pages 848–854