کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3144536 1406816 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of Candida albicans biofilm development by unencapsulated Enterococcus faecalis cps2
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Inhibition of Candida albicans biofilm development by unencapsulated Enterococcus faecalis cps2
چکیده انگلیسی

Background/purposeIn the oral environment, Candida albicans interacts with many bacteria, including Enterococcus faecalis. We investigated the susceptibility of C. albicans biofilm development to the presence of unencapsulated E. faecalis cps2 in comparison with reference strains (E. faecalis ATCC 29212) or their respective spent medium (collected at 6 hours).Material and methodsCrystal violet stain was used to measure the total biofilm mass, whereas quantitative real-time polymerase chain reaction was used to analyze the change in expression of the mRNA of hypha morphology (ALS1 and ALS3) and biofilm maturation (EFB1).ResultsAt the intermediate stage, C. albicans resisted the presence of each E. faecalis strain tested and their spent medium. However, at the maturation stage, the unencapsulated strain was stronger in reducing C. albicans biofilms than the reference strain (P < 0.05). At this maturation stage, the transcription levels of each gene tested decreased in the presence of either E. faecalis strains or their respective spent medium. The unencapsulated strain was more pronounced in reducing ALS1/ALS3 expression, whereas the respective spent medium had a similar capability to restrict the expression of EFB1.ConclusionThis study showed, the unencapsulated strain is more effective in inhibiting C. albicans biofilm development compared with the reference strains. In contrast, the secreted molecules produced by each strain tested are necessary in controlling the growths of C. albicans biofilm.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dental Sciences - Volume 11, Issue 3, September 2016, Pages 323–330
نویسندگان
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