کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3164391 1198787 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oncolytic Vaccinia virus and radiotherapy in head and neck cancer
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Oncolytic Vaccinia virus and radiotherapy in head and neck cancer
چکیده انگلیسی

SummaryObjectiveOncolytic forms of attenuated Vaccinia virus are now in clinical development, assessing the compatibility of this novel treatment with radiotherapy may reveal exploitable synergistic relationships.Materials and methodsIn vitro analyses of cell killing, cell cycle effects and caspase activation were carried out on HN3, HN5, CAL27, Detroit, SIHN5B, and PJ41 cells. In vivo studies of the virus and X-radiation were performed on H&N xenografts in CD1 nude mice.ResultsCell killing in vitro was demonstrated to be dose- and time-dependent. Infection causes an increase in S-phase and sub-G1 cells. A dose dependent increase in active caspase-3 indicated induction of apoptosis. Xenografts injected with Vaccinia stabilised and frequently completely regressed. Combination with radiation generated additional cell death, induction of caspase activity and in vivo further improved long term regression rates.ConclusionsThese data support continued exploration of this therapy combination and indicates potential for clinical trials in head and neck cancer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Oral Oncology - Volume 49, Issue 2, February 2013, Pages 108–118
نویسندگان
, , , , , , , , , , , , , , ,