Chronotype changes during puberty depend on gonadal hormones in the slow-developing rodent, Octodon degus

During puberty, human adolescents develop a later chronotype, exhibiting a delay in the timing of rest and activity as well as other daily physiological rhythms. The purpose of this study was to determine whether similar changes in chronotype occur during puberty in a laboratory rodent species, and, if so, to determine whether they are due to pubertal hormones acting on the circadian timekeeping system. To test this hypothesis, we carefully tracked daily activity rhythms across puberty in the slow-developing rodent Octodon degus. We confirmed that male degus showed a large reorganization of activity rhythms that correlated with secondary sex development during puberty, including a loss of bimodality and a 3–5 h phase-advance. Similar to humans, this circadian reorganization showed distinct sex differences, with females showing little change during puberty in two separate experiments. Prepubertal gonadectomy (GDX) eliminated the changes, whereas SHAM gonadectomy had little impact. Therefore, gonadal hormones are likely to play a role in pubertal changes in chronotype in this rodent species. Using evidence from a variety of species, including our recent studies in the rat, we conclude that chronotype changes during puberty are a well-demonstrated phenomenon in mammals.

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► The circadian system continues to develop during puberty.
► Peripubertal degus had activity rhythms that were more phase-delayed and bimodal than those of adults.
► Male degus showed a consolidation and phase-advance of activity rhythms with maturation.
► Female degus and castrates maintained less consolidated, delayed rhythms.
► Gonadal hormones are likely to play an essential role in pubertal circadian development.