کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3257147 | 1207394 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: B-cell exposure to self-antigen induces IL-10 producing B cells as well as IL-6- and TNF-α-producing B-cell subsets in healthy humans B-cell exposure to self-antigen induces IL-10 producing B cells as well as IL-6- and TNF-α-producing B-cell subsets in healthy humans](/preview/png/3257147.png)
Human B cells are able to secrete IL-10 after stimulation with mitogens, but their ability to produce IL-10 and regulate T-cell responses after stimulation with self-antigens is unclear. We co-cultured thyroglobulin-pulsed B cells from healthy donors with autologous T cells and observed production of IL-10 and TGF-β, in addition to TNF-α and IL-6. Pulsing with foreign antigen, tetanus toxoid (TT), induced a Th1-response with minimal IL-10 production. After thyroglobulin-pulsing, 1.10 ± 0.50% of B cells and 1.00 ± 0.20% of CD4+ T cells produced IL-10, compared to 0.29 ± 0.19% of B cells (P = 0.01) and 0.13 ± 0.15% of CD4+ T cells (P = 0.006) following TT-pulsing. Thyroglobulin-stimulated, IL-10-secreting B cells were enriched within CD5+ and CD24high cells. While thyroglobulin-pulsed B cells induced only modest proliferation of CD4+ T cells, B cells pulsed with TT induced vigorous proliferation. Thus, B cells mediate self-antigen-specific IL-10, TNF-α and IL-6 production in co-cultures with T cells and contribute actively to these cytokine secretions.
► Pulsing B cells with thyroglobulin induced IL-10 production.
► The IL-10 producing B cells were enriched within CD5+ and CD24high cells.
► Co-cultivated TG-pulsed B cells with autologous T cells produced IL-10, TNF-α and IL-6.
Journal: Clinical Immunology - Volume 145, Issue 1, October 2012, Pages 1–10