کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3257149 | 1207394 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Peripheral accumulation of newly produced T and B lymphocytes in natalizumab-treated multiple sclerosis patients Peripheral accumulation of newly produced T and B lymphocytes in natalizumab-treated multiple sclerosis patients](/preview/png/3257149.png)
The anti-α4 monoclonal antibody natalizumab inhibits lymphocyte extravasation into the central nervous system and increases peripheral T and B lymphocytes in multiple sclerosis patients. To investigate whether the lymphocyte accumulation was due to a higher lymphocyte production, an altered homeostasis, or a differential transmigration of lymphocyte subsets through endothelia, T-cell receptor excision circles and kappa-deleting recombination excision circles were quantified before and after treatment, T-cell receptor repertoire was analyzed by spectratyping, and T- and B-lymphocyte subset migration was studied using transwell coated with vascular and lymphatic endothelial cells. We found that the number of newly produced T and B lymphocytes is increased because of a high release and of a low propensity of naïve subsets to migrate across endothelial cells. In some patients this resulted in an enlargement of T-cell heterogeneity. Because new lymphocyte production ensures the integrity of immune surveillance, its quantification could be used to monitor natalizumab therapy safety.
► Newly produced T and B lymphocytes increase in natalizumab-treated MS patients.
► Increased thymic output in some patients results in a broader T-cell heterogeneity.
► Accumulation of new naïve T and B cells may balance the expansion of memory cells.
► Naïve cells show a lower capacity to migrate across endothelia than memory cells.
► The accumulation of new lymphocytes contributes to immune cell renewal in MS.
Journal: Clinical Immunology - Volume 145, Issue 1, October 2012, Pages 19–26