کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3285852 | 1209245 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment of Differentiation and Progression of Hepatic Tumors Using Array-Based Comparative Genomic Hybridization
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کلمات کلیدی
PACSSCHCACGHArray-CGHHCC - HCCBAC - LACHepatocellular adenoma - آدنوم خونسردیstandard saline citrate - استاندارد سدیم سیتراتfluorescence in situ hybridization - فلورسانس در هیبریداسیون در محلFish - ماهیComparative genome hybridization - هیبریداسیون ژنوم مقایسه شدهArray-based comparative genomic hybridization - هیبریداسیون ژنومی مقایسه ای مبتنی بر آرایهHepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)bacterial artificial chromosome - کروموزوم مصنوعی باکتریایی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: To gain more information about the molecular mechanisms leading to dedifferentiation of hepatocellular adenoma (HCA) and hepatocellular carcinoma (HCC), high-resolution array-based comparative genomic hybridization (array-CGH) was performed on 24 cases of HCC and 10 cases of HCA. Methods: DNA chips containing 6251 individual bacterial artificial chromosome/plasmid artificial chromosome clones were used. They allowed for a genome-wide resolution of 1 Mb and an even higher resolution of up to 100 kb for chromosome regions recurrently involved in human tumors and for regions containing known tumor-suppressor genes and oncogenes. Results: Copy number changes on the genomic scale were found by array-based comparative genomic hybridization in all cases. In HCC, gains of chromosomal regions 1q (91.6%), and 8q (58.3%), and losses of 8p (54%) were found most frequently. Hierarchic cluster analysis branched all HCA from HCC. However, in 2 adenomas with a known history of glycogenosis type I and adenomatosis hepatis gains of 1q were found, too. The critically gained region was narrowed down to bands 1q22-23. Although no significant differences in the mean number of chromosomal aberrations were seen between adenomas and well-differentiated carcinomas (2.7 vs 4.6), a significant increase accompanied the dedifferentiation of HCC (14.1 in HCC-G2 and 16.3 in HCC-G2/3; P < .02). Dedifferentiation of HCC also was correlated closely to losses of 4q and 13q (P <.001 and <.005, respectively). Conclusions: The increased chromosomal instability during dedifferentiation of HCC leads to an accumulation of structural chromosomal aberrations and losses and gains of defined chromosome regions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Gastroenterology and Hepatology - Volume 4, Issue 10, October 2006, Pages 1283-1291
Journal: Clinical Gastroenterology and Hepatology - Volume 4, Issue 10, October 2006, Pages 1283-1291
نویسندگان
Doris Steinemann, Britta Skawran, Thomas Becker, Marcel Tauscher, Anja Weigmann, Luzie Wingen, Sarah Tauscher, Tanja Hinrichsen, Sabine Hertz, Peer Flemming, Jacobus Flik, Birgit Wiese, Hans Kreipe, Peter Lichter, Brigitte Schlegelberger, Ludwig Wilkens,