Differential Expression of Ly6C and T-bet Distinguish Effector and Memory Th1 CD4+ Cell Properties during Viral Infection

SummaryCD4+ T cells differentiate into multiple effector types, but it is unclear how they form memory T cells during infection in vivo. Profiling virus-specific CD4+ T cells revealed that effector cells with T helper 1 (Th1) or T follicular helper (Tfh) cell characteristics differentiated into memory cells, although expression of Tfh cell markers declined over time. In contrast to virus-specific effector CD8+ T cells, increased IL-7R expression was not a reliable marker of CD4+ memory precursor cells. However, decreased Ly6C and T-bet (Tbx21) expression distinguished a subset of Th1 cells that displayed greater longevity and proliferative responses to secondary infection. Moreover, the gene expression profile of Ly6CloT-betint Th1 effector cells was virtually identical to mature memory CD4+ T cells, indicating early maturation of memory CD4+ T cell features in this subset during acute viral infection. This study provides a framework for memory CD4+ T cell development after acute viral infection.

Graphical AbstractFigure optionsDownload high-quality image (257 K)Download as PowerPoint slideHighlights
► Increased IL-7R expression does not mark CD4+ memory precursor cells
► T-bet acts in a graded manner to regulate formation of Th1 and Tfh cell subsets
► Ly6CloT-betint Th1 effector CD4+ cells have enhanced longevity and recall responses
► Ly6CloT-betint Th1 effector and memory CD4+ cells share similar gene expression