Early Th1 Cell Differentiation Is Marked by a Tfh Cell-like Transition

SummaryFollicular helper T (Tfh) cells comprise an important subset of helper T cells; however, their relationship with other helper lineages is incompletely understood. Herein, we showed interleukin-12 acting via the transcription factor STAT4 induced both Il21 and Bcl6 genes, generating cells with features of both Tfh and Th1 cells. However, STAT4 also induced the transcription factor T-bet. With ChIP-seq, we defined the genome-wide targets of T-bet and found that it repressed Bcl6 and other markers of Tfh cells, thereby attenuating the nascent Tfh cell-like phenotype in the late phase of Th1 cell specification. Tfh-like cells were rapidly generated after Toxoplasma gondii infection in mice, but T-bet constrained Tfh cell expansion and consequent germinal center formation and antibody production. Our data argue that Tfh and Th1 cells share a transitional stage through the signal mediated by STAT4, which promotes both phenotypes. However, T-bet represses Tfh cell functionalities, promoting full Th1 cell differentiation.

Graphical AbstractFigure optionsDownload high-quality image (145 K)Download as PowerPoint slideHighlights
► STAT4 drives Il21 and Bcl6 genes to create a cell with Tfh and Th1 cell features
► IFN-γ and T-bet attenuates the Tfh cell-like phenotype at later stage of the specification
► The Bcl6 locus remains accessible in fully polarized Th1 cells
► T-bet limits Tfh and germinal center B cell generation during T. gondii infection