CD45-Csk Phosphatase-Kinase Titration Uncouples Basal and Inducible T Cell Receptor Signaling during Thymic Development

SummaryThe kinase-phosphatase pair Csk and CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn. T cell receptor (TCR) signaling and thymic development require CD45 expression but proceed constitutively in the absence of Csk. Here, we show that relative titration of CD45 and Csk expression reveals distinct regulation of basal and inducible TCR signaling during thymic development. Low CD45 expression is sufficient to rescue inducible TCR signaling and positive selection, whereas high expression is required to reconstitute basal TCR signaling and beta selection. CD45 has a dual positive and negative regulatory role during inducible but not basal TCR signaling. By contrast, Csk titration regulates basal but not inducible signaling. High physiologic expression of CD45 is thus required for two reasons—to downmodulate inducible TCR signaling during positive selection and to counteract Csk during basal TCR signaling.

► CD45 has a dual positive and negative regulatory role during inducible TCR signaling
► CD45 has only a positive regulatory role in beta selection and basal TCR signaling
► High physiologic amounts of CD45 inhibit inducible TCR signaling in DP thymocytes
► High physiologic amounts of CD45 are required to counteract Csk during basal signaling