کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3353790 1216889 2009 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential Contribution of Chemotaxis and Substrate Restriction to Segregation of Immature and Mature Thymocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Differential Contribution of Chemotaxis and Substrate Restriction to Segregation of Immature and Mature Thymocytes
چکیده انگلیسی

SummaryT cell development requires sequential localization of thymocyte subsets to distinct thymic microenvironments. To address mechanisms governing this segregation, we used two-photon microscopy to visualize migration of purified thymocyte subsets in defined microenvironments within thymic slices. Double-negative (CD4−8−) and double-positive (CD4+8+) thymocytes were confined to cortex where they moved slowly without directional bias. DP cells accumulated and migrated more rapidly in a specialized inner-cortical microenvironment, but were unable to migrate on medullary substrates. In contrast, CD4 single positive (SP) thymocytes migrated directionally toward the medulla, where they accumulated and moved very rapidly. Our results revealed a requisite two-step process governing CD4 SP cell medullary localization: the chemokine receptor CCR7 mediated chemotaxis of CD4 SP cells towards medulla, whereas a distinct pertussis-toxin sensitive pathway was required for medullary entry. These findings suggest that developmentally regulated responses to both chemotactic signals and specific migratory substrates guide thymocytes to specific locations in the thymus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 31, Issue 6, 18 December 2009, Pages 986–998
نویسندگان
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