کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3354204 | 1216918 | 2007 | 10 صفحه PDF | دانلود رایگان |

SummaryT cells interact with dendritic cells (DCs) for periods lasting from minutes to hours. However, a causal link between the duration of this interaction and the efficiency of T cell activation has not been established in vivo. Employing intravital two-photon imaging, we manipulated T cell-DC interactions in real time and found that the first T cell-DC encounter often resulted in a long-lived interaction. Moreover, the cessation of T cell receptor-major histocompatibility complex signals promoted cellular dissociation, suggesting that antigen availability on DCs regulates contact duration. Finally, at least 6 hr of in vivo T cell-DC interaction were required for naive CD4+ T cells to undergo clonal expansion. These results establish the importance of prolonged T cell-DC interactions for efficient CD4+ T cell activation in vivo.
Journal: - Volume 27, Issue 4, 26 October 2007, Pages 625–634