A monoclonal antibody for the detection of SNAP/CLIP-tagged proteins

SNAP/CLIP-tag technology is a novel approach that allows tagged proteins to be covalently coupled to diverse labels, such as fluorochromes and particles, using a convenient and specific enzymatic reaction. A monoclonal antibody (mAb) that binds to the SNAP/CLIP-tag would be useful to determine labeling efficiency, and to achieve reproducible detection in a variety of experimental formats. We therefore generated the murine mAb M2D11 by standard immunization and hybridoma technology. M2D11 binds to both the SNAP- and the CLIP-tag in either the coupled or uncoupled configurations and can be detected in the context of ELISA, flow cytometry, immunohistochemistry and western blot. The new antibody increases the versatility of the SNAP-tag technology by enabling the detection of tagged proteins using conventional immunological methods and widely available secondary antibodies.

► Generation of a murine monoclonal antibody (M2D11) against the SNAP- and CLIP-tag.
► M2D11 can be used to detect both tags with and without a coupled substrate.
► M2D11 recognizes the SNAP- and CLIP-tag in their native and denatured form.
► M2D11 can be used for ELISA, flow cytometry, immunohistochemistry and western blotting.