کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3355576 1591568 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Th2-Th1 shift with the multiantigenic formulation TERAVAC-HIV-1 in Balb/c mice
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Th2-Th1 shift with the multiantigenic formulation TERAVAC-HIV-1 in Balb/c mice
چکیده انگلیسی

In chronic HIV infection a progressive Th1 to Th2/Th0 cytokine-profile shift is related to disease progression. One of the possible benefits of a therapeutic vaccination might be to counterbalance this phenomenon to allow viral replication control under a Th1-type immune response. TERAVAC-HIV-1 is a multiantigenic formulation vaccine candidate against HIV-1 which comprises the recombinant protein CR3 that contains T cell epitopes and the surface and nucleocapsid antigens of Hepatitis B Virus (HBV). Previous studies showed that such virus like particles of the HBV provide a Th1 adjuvant effect. The present studies examined the capacity of TERAVAC to elicit a Th1 response in the presence of an ongoing HIV-specific Th2-type response in Balb/c mice. To examine this issue, we injected subcutaneously the animals with CR3 or viral lysate in alum which resulted in a Th2-type response. The CR3-specific Th2-type response was verified by induction of IL-4 and IL-10 secretion in ex vivo stimulated splenocytes without secretion of IFN-γ and IgG2a antibodies in serum. Further subcutaneous and simultaneous subcutaneous-nasal immunizations of the same mice with TERAVAC promoted IFN-γ secretion and production of IgG2a antibodies in accordance with a Th1-type response. This result suggests a therapeutic benefit of this vaccine candidate in the restoration of the Th1-type HIV-specific cellular response in seropositive patients.


► Induction of a HIV-specific Th2-type cytokine response in Balb/c mice.
► Vaccine candidate TERAVAC induced a Th2-Th1 shift.
► SC and simultaneous IN and SC inoculations elicited IFN-γ-secreting cells.
► They also promoted IgG2a antibody production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 149, Issues 1–2, January 2013, Pages 77–84
نویسندگان
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