کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3355613 | 1217194 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Melanoma cell-derived exosomes alter macrophage and dendritic cell functions in vitro Melanoma cell-derived exosomes alter macrophage and dendritic cell functions in vitro](/preview/png/3355613.png)
To clarify controversies in the literature of the field, we have purified and characterized B16F1 melanoma cell derived exosomes (mcd-exosomes) then we attempted to dissect their immunological activities. We tested how mcd-exosomes influence CD4+ T cell proliferation induced by bone marrow derived dendritic cells; we quantified NF-κB activation in mature macrophages stimulated with mcd-exosomes, and we compared the cytokine profile of LPS-stimulated, IL-4 induced, and mcd-exosome treated macrophages. We observed that mcd-exosomes helped the maturation of dendritic cells, enhancing T cell proliferation induced by the treated dendritic cells. The exosomes also activated macrophages, as measured by NF-κB activation. The cytokine and chemokine profile of macrophages treated with tumor cell derived exosomes showed marked differences from those induced by either LPS or IL-4, and it suggested that exosomes may play a role in the tumor progression and metastasis formation through supporting tumor immune escape mechanisms.
► B16 melanomas produce exosomes of 30–70 nm diameter.
► The exosomes help the functional maturation of allogeneic dendritic cells.
► The exosomes activate macrophages.
► The cytokine profile of exosome-induced macrophages is different from those of LPS- or IL-4-treated macrophages.
Journal: Immunology Letters - Volume 148, Issue 1, November–December 2012, Pages 34–38