کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3356304 | 1217253 | 2007 | 8 صفحه PDF | دانلود رایگان |
Humanized antibody-based treatment modalities represent an active area of investigation. Included in these strategies are passive administrations of monoclonal antibodies, which recognize tumor necrosis factor alpha (TNF-α). However, several problems associated with these types of treatment strategies have been reported in the literature. We attempted to address the issue related to unresponsiveness to infliximab that might be induced by anti-idiotype response to the passively administered humanized monoclonal antibody.The characteristics and functional importance of antibodies to infliximab (ATI) were investigated in human sera. We studied the binding characteristics of ATI to infliximab, TNF-α Receptor-I (RI, p55) and Receptor-II (RII, p75). In addition, cytotoxicity effect on L929 cells and blocking effects on the binding of TNF-α with infliximab and etanercept were also analyzed.On the basis of the results obtained from the experiments, it seems that the target epitope for ATI is related with somewhere else not residing in the region capable of generating “mirror image”. The results presented indicate that ATI does not mimic the functional characteristics of TNF-α. However, ATI inhibited the binding properties of infliximab to TNF-α.
Journal: Immunology Letters - Volume 111, Issue 2, 15 August 2007, Pages 84–91