کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3356308 1217253 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-α and oxLDL
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-α and oxLDL
چکیده انگلیسی

BackgroundRecent findings indicate that atherosclerosis, a chronic inflammatory process, might start during childhood. Nevertheless, the expression of inflammation-related molecules of endothelial cell isolated from healthy neonates with a strong family history of myocardial infarction (SFHMI) has been rarely analyzed.MethodsHuman umbilical vein endothelial cells (HUVECs) from children with SFHMI were assessed for the expression of CD40 and CD40L, in the presence of TNF-α and oxLDL. The intracellular content of CD80, CXCL8 and tissue factor by HUVECs stimulated with a CD40 agonist monoclonal antibody as well as monocytes/lymphocyte adhesion to TNF-α-stimulated HUVECs was also evaluated.ResultsThe basal expression of CD40 and CD40L was higher in SFHMI-positive HUVECs in comparison to controls. TNF-α and oxLDL upregulated the expression of CD40 and CD40L in SFHMI versus control HUVECs (p < 0.001). The intracellular expression of CXCL8, tissue factor and CD80 was also higher than in controls, and the adhesion of lymphocyte- and monocyte-like cells augmented upon TNF-α stimulation.ConclusionsIt is possible that the modifications observed in the SFHMI-positive HUVECs, all of them relevant to the atherosclerosis process, may lead to early inflammatory reactions, thus contributing to the premature initiation of atherosclerotic lesions in these children.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 111, Issue 2, 15 August 2007, Pages 116–123
نویسندگان
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