Early posttransplant changes in circulating endothelial microparticles in patients with kidney transplantation

• We analyzed changes in endothelial microparticles (EMPs) after kidney transplantation.
• EMPs change after kidney transplantation in selected patient population.
• EMPs show different dynamics in peritubular capillary C4d positive or negative biopsies.

BackgroundEndothelial microparticles (EMPs) are membrane vesicles shed from endothelial cell in response to injury, activation or apoptosis. Kidney transplantation (KTx) is the treatment of choice for patients with end stage kidney disease (ESKD). The aim of this study was to analyze changes in EMP and serum creatinine (SCr) in patients following KTx.MethodsBlood was periodically collected from patients before (pre-KTx) and after KTx for two months. EMPs were identified as CD31+/CD42b− microparticles and quantified by fluorescence-activated cell scanning.ResultsThis study included 213 KTx, 14 kidney/pancreas (KPTx) recipients and 60 healthy donors prior to donation. The recipients were divided into 5 groups based on the cause of ESKD. No differences in the quantity of circulating EMP were seen in the pre-KPTx or KTx recipient sera and healthy donor sera. Patients with ESKD secondary to diabetes mellitus, obstructive/inherited kidney disease and autoimmune disease had a decrease in both circulating EMP and SCr by day 60 after KTx.ConclusionReduction in both circulating EMP and SCr was seen after kidney KTx in patients with selective ESKD.